Rapid Discovery of the Potential Toxic Compounds in Polygonum multiflorum by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis

作者：Lifeng Han, Piao Wang, Yulan Wang, Qianyu Zhao, Fang Zheng, Zhiying Dou, Wenzhi Yang, Limin Hu, Caixiang Liu · 发表于：Frontiers in Pharmacology · 年份：2019 · DOI：10.3389/fphar.2019.00329 · 被引用次数：37 · 研究领域：Phytochemistry and biological activity of medicinal plants、Ginseng Biological Effects and Applications、Pharmacological Effects of Natural Compounds

The dry roots of polygonum multiflorum (PM), involving both the raw and processed materials, are widely used as the traditional Chinese medicine for treating various diseases in China. Hepatotoxicity has been occasionally reported in patients who consume PM. Unfortunately, no definite criteria are currently available regarding the processing technology of PM. In this work, we were aimed to investigate the variations of PM metabolite profiles induced by different processing technologies by feat of UHPLC/Q-Orbitrap-MS and multivariate statistical analysis, and to discover the potential toxic compounds by correlating the cytotoxicity of L02 cell with the contents of metabolites in raw and processed PM samples. We could identify two potential toxic compounds, emodin-8-O-glucoside and torachrysone-O-hexose, which could be selected as the toxic markers to evaluate different processing methods. The results indicated all processed PM samples could decrease the cytotoxicity on L02 cell. The best processing technology for PM process was to steam PM in black soybean decoction (BD-PM) for 24 hours.