Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine

作者：Matthieu Pierre Platre, Vincent Bayle, Laia Armengot, Joseph Bareille, María Mar Marquès‐Bueno, Audrey Creff, Lilly Maneta-Peyret, Jean-Bernard Fiche, Marcelo Nöllmann, Christine Miège, Patrick Moreau, Alexandre Martinière, Yvon Jaillais · 发表于：Science · 年份：2019 · DOI：10.1126/science.aav9959 · 被引用次数：262 · 研究领域：Photosynthetic Processes and Mechanisms、Plant Molecular Biology Research、Plant Reproductive Biology

Live superresolution single-molecule imaging revealed that the protein Rho of Plants 6 (ROP6) is stabilized by phosphatidylserine into plasma membrane nanodomains, which are required for auxin signaling. Our experiments also revealed that the plasma membrane phosphatidylserine content varies during plant root development and that the level of phosphatidylserine modulates the quantity of ROP6 nanoclusters induced by auxin and hence downstream signaling, including regulation of endocytosis and gravitropism. Our work shows that variations in phosphatidylserine levels are a physiological process that may be leveraged to regulate small GTPase signaling during development.