OsCIPK7 point‐mutation leads to conformation and kinase‐activity change for sensing cold response

作者：Dajian Zhang, Xiaoyu Guo, Yunyuan Xu, Hao Li, Liang Ma, Xuefeng Yao, Yuxiang Weng, Yan Guo, Chunming Liu, Kang Chong · 发表于：Journal of Integrative Plant Biology · 年份：2019 · DOI：10.1111/jipb.12800 · 被引用次数：70 · 研究领域：Signaling Pathways in Disease、Plant Stress Responses and Tolerance、Heat shock proteins research

Calcineurin B-like interacting protein kinases (CIPKs) play important roles via environmental stress. However, less is known how to sense the stress in molecular structure conformation level. Here, an OsCIPK7 mutant via TILLING procedure with a point mutation in the kinase domain showed increased chilling tolerance, which could be potentially used in the molecular breeding. We found that this point mutation of OsCIPK7 led to a conformational change in the activation loop of the kinase domain, subsequently with an increase of protein kinase activity, thus conferred an increased tolerance to chilling stress.