Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer

作者：Jiantong Dong, Ryan Y. Zhang, Na Sun, Matthew D. Smalley, Zipeng Wu, Anqi Zhou, Shih‐Jie Chou, Yu Jen Jan, Peng Yang, Lirong Bao, Dongping Qi, Xinghong Tang, Patrick Tseng, Yue Hua, Dianwen Xu, Rueihung Kao, Meng Meng, Xirun Zheng, Ying Liu, Tatyana Vagner, Xiaoshu Chai, Dongjing Zhou, Mengyuan Li, Shih‐Hwa Chiou, Guangjuan Zheng, Dolores Di Vizio, Vatche G. Agopian, Edwin Melencio Posadas, Steven John Jonas, Shin‐Pon Ju, Paul S. Weiss, Meiping Zhao, Hsian‐Rong Tseng, Yazhen Zhu · 发表于：ACS Applied Materials & Interfaces · 年份：2019 · DOI：10.1021/acsami.9b01406 · 被引用次数：75 · 研究领域：Extracellular vesicles in disease、MicroRNA in disease regulation、Anodic Oxide Films and Nanostructures

Tumor-derived extracellular vesicles (EVs) present in bodily fluids are emerging liquid biopsy markers for non-invasive cancer diagnosis and treatment monitoring. Because the majority of EVs in circulation are not of tumor origin, it is critical to develop new platforms capable of enriching tumor-derived EVs from the blood. Herein, we introduce a biostructure-inspired NanoVilli Chip, capable of highly efficient and reproducible immunoaffinity capture of tumor-derived EVs from blood plasma samples. Anti-EpCAM-grafted silicon nanowire arrays were engineered to mimic the distinctive structures of intestinal microvilli, dramatically increasing surface area and enhancing tumor-derived EV capture. RNA in the captured EVs can be recovered for downstream molecular analyses by reverse transcription Droplet Digital PCR. We demonstrate that this assay can be applied to monitor the dynamic changes of ROS1 rearrangements and epidermal growth factor receptor T790M mutations that predict treatment responses and disease progression in non-small cell lung cancer patients.