Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring

作者：A Fayomi, Karen A. Peters, Meena Sukhwani, Hanna Pulaski, Gunapala Shetty, Marvin L. Meistrich, Lisa A. Houser, Nicola Robertson, Victoria H. J. Roberts, Cathy Ramsey, Carol Hanna, Jon D. Hennebold, Ina Dobrinski, Kyle E. Orwig · 发表于：Science · 年份：2019 · DOI：10.1126/science.aav2914 · 被引用次数：336 · 研究领域：Sperm and Testicular Function、Reproductive Biology and Fertility、Reproductive Health and Technologies

Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.