Monitoring autophagy in live cells with a fluorescent light-up probe for G-quadruplex structures

作者：Hongbo Chen, Hongxia Sun, Suge Zhang, Wenpeng Yan, Qian Li, Aijiao Guan, Junfeng Xiang, Meirong Liu, Yalin Tang · 发表于：Chemical Communications · 年份：2019 · DOI：10.1039/c9cc01263j · 被引用次数：31 · 研究领域：DNA and Nucleic Acid Chemistry、RNA Interference and Gene Delivery、Advanced biosensing and bioanalysis techniques

Monitoring autophagy can provide valuable insights into understanding human pathological mechanisms, developing novel drugs, and exploring autophagy control approaches. Here, we proposed a new strategy to specifically monitor autophagy by lighting up the G-quadruplex structures entering autolysosomes. Based on this strategy, we designed a small-molecule fluorescent probe for autophagy imaging. This work not only opens up a new way for developing autophagy probes, but also provides an effective tool for autophagy research.