Size effect of nano-hydroxyapatite on proliferation of odontoblast-like MDPC-23 cells

作者：Na Li, Gang Wu, Hua Yao, Ruikang Tang, Xinhua Gu, Chengwei Tu · 发表于：Dental Materials Journal · 年份：2019 · DOI：10.4012/dmj.2018-155 · 被引用次数：19 · 研究领域：Bone Tissue Engineering Materials、Dental materials and restorations、Dental Implant Techniques and Outcomes

Nano-hydroxyapatite (nano-HAP) is supposed to be a promising candidate for apatite substitute in hard tissue engineering. We aimed to investigate the effect of nano-HAP particles on the proliferation of odontoblast-like MDPC-23 cells compared with conventional hydroxyapatite (c-HAP). HAP in diameter of ~20 nm (np20), ~70 nm (np70) and ~200 nm (c-HAP) were synthesized and characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM). Inverted microscope and MTT assay were used to detect the morphology and proliferation rate of MDPC-23 cells; TEM was used to reveal the internalization of HAP. We found that nano-HAP (np20 and np70), especially np20 expressed obvious growth-promoting effect on MDPC-23 cells compared with c-HAP, which caused the most vacuole in MDPC-23 cells. These results suggest that nano-HAP may be an optimal choice of apatite substitute for MDPC-23 cells on the aspect of cell proliferation.