Core Regulatory RNA Molecules Identified in Articular Cartilage Stem/Progenitor Cells During Osteoarthritis Progression

作者：Shuai Zhang, Qier An, Peilin Hu, Xiaomin Wu, Xiaohua Pan, Wenjin Peng, Rikang Wang, Jingyi Gan, Di Chen, Zhen Li, Tianfu Wang, Guangqian Zhou · 发表于：Epigenomics · 年份：2019 · DOI：10.2217/epi-2018-0212 · 被引用次数：27 · 研究领域：Cancer-related molecular mechanisms research、Osteoarthritis Treatment and Mechanisms、Circular RNAs in diseases

Aim: To assess cartilage-derived stem/progenitor cells (CSPCs) in osteoarthritis (OA) by employing mRNA-miRNA-circRNA-lncRNA network biology approach. Methods: Differentially expressed (DE) RNAs in CSPCs from 2-/4-/8-month-old STR/Ort and CBA mice were identified to construct networks via RNA sequencing. Results: Compared with age-matched CBA mice, 4-/8-month-old STR/Ort mice had cartilage lesions and their CSPCs exhibited lower proliferative and differentiation capacity (decreased CD44 and CD90), and identified 7082 DE RNAs in STR/Ort mice were associated with strain differences or OA progression. OA-related core RNAs were identified via the networks constructed with the predominant DE RNAs, which were involved in the signaling pathways (NF-κB/MAPK/Hippo/Wnt/TGF-β/cytoskeleton organization). The core RNAs (miR-322-5p/miR-493-5p/miR-378c/CPNE1/Cdh2/PRDM16/CTGF/NCAM1) were validated in CSPCs from OA patients. Conclusion: RNA-based networks identifying core RNAs and signaling pathways contribute to CSPC-dependent OA mechanisms.