<p>Overexpressed long noncoding RNA <em>TUG1 </em>affects the cell cycle, proliferation, and apoptosis of pancreatic cancer partly through suppressing RND3 and MT2A</p>

作者：Bingqing Hui, Yetao Xu, Benpeng Zhao, Hao Ji, Zhonghua Ma, Shufen Xu, Zhenyu He, Keming Wang, Jianwei Lu · 发表于：OncoTargets and Therapy · 年份：2019 · DOI：10.2147/ott.s188396 · 被引用次数：37 · 研究领域：Cancer-related molecular mechanisms research、Pancreatic and Hepatic Oncology Research、MicroRNA in disease regulation

Background: Long noncoding RNAs (lncRNAs) are involved in various human diseases, including cancers. However, their mechanisms remain undocumented. We investigated alterations in lncRNA that may be related to pancreatic cancer (PC) through analysis of microarray data. Methods: In the present study, quantitative real-time PCR analysis was used to examine the expression of taurine upregulated 1 ( TUG1 ) in PC tissue samples and PC cell lines. In PC cell lines, MTT assays, colony formation assays, and flow cytometry were used to investigate the effects of TUG1 on proliferation, cell cycle regulation, and apoptosis. Moreover, we established a xenograft model to assess the effect of TUG1 on tumor growth in vivo. The molecular mechanism of potential target genes was detected through nuclear separation experiments, RNA immunoprecipitation (RIP), chromatin immunoprecipitation assays (ChIP), and other experimental methods. Results: The findings suggest that the abnormally high expression of TUG1 in PC tissues was associated with tumor size and pathological stage. Knockdown of TUG1 blocked the cell cycle and accelerated apoptosis, thereby inhibiting the proliferation of PC cells. In addition, RIP experiments showed that TUG1 can recruit enhancer of zeste homolog 2 (EZH2) to the promoter regions of Rho family GTPase 3 ( RND3 ) and metallothionein 2A ( MT2A ) and inhibit their expression at the transcriptional level. Furthermore, ChIP experiments demonstrated that EZH2 could bind to the ...