Dynamic Development of Fecal Microbiome During the Progression of Diabetes Mellitus in Zucker Diabetic Fatty Rats

作者：Wen Zhou, Huiying Xu, Libin Zhan, Xiaoguang Lu, Lijing Zhang · 发表于：Frontiers in Microbiology · 年份：2019 · DOI：10.3389/fmicb.2019.00232 · 被引用次数：89 · 研究领域：Gut microbiota and health、Diet and metabolism studies、Clostridium difficile and Clostridium perfringens research

Background: Although substantial efforts have been made to link the gut microbiota to type 2 diabetes, dynamic changes in the fecal microbiome under the pathological conditions of diabetes have not been investigated. Methods：Four male Zucker diabetic fatty (ZDF) rats were received Purina 5008 chow [protein=23.6%, Nitrogen-Free Extract(by difference)=50.3%, fiber(crude)=3.3%, ash=6.1%, fat(ether extract)=6.7%, and fat(acid hydrolysis)=8.1%] for 8 weeks. A total of 32 stool samples were collected from week 8 to week 15 in four rats. To decipher the microbial populations in these samples, we used a 16S rRNA gene sequencing approach. Results: Microbiome analysis showed that the changes in the fecal microbiome were associated with age and disease progression. In all the stages from 8 to 15 weeks of rats, phyla Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria primarily dominated the fecal microbiome. Although Lactobacillus and Turicibacter were the predominant genera in 8- to 10-week-old rats, Bifidobacterium, Lactobacillus, Ruminococcus, and Allobaculum were the most abundant genera in 15-week-old rats. Of interest, compared to the earlier weeks, relatively greater diversity (at the genus level) was observed at 10 weeks of age. Although the microbiome of 12-week-old rats had the maximum diversity, the diversity in 13–15-week-old rats was reduced. Spearman’s correlation analysis showed that F/B was negatively correlated with age. Random blood glucose was negatively cor...