IL-4-polarized BV2 microglia cells promote angiogenesis by secreting exosomes

作者：Yan Tian, Pei Zhu, Shanshan Liu, Zheng Jin, Dong Li, Heng Zhao, Xun Sheng Zhu, Chang Shu, Dongmei Yan, Zehua Dong · 发表于：Advances in Clinical and Experimental Medicine · 年份：2019 · DOI：10.17219/acem/91826 · 被引用次数：100 · 研究领域：Neuroinflammation and Neurodegeneration Mechanisms、Extracellular vesicles in disease、MicroRNA in disease regulation

BACKGROUND: The microglia cell transfer has been shown to play a protective role in ischemic stroke. Microglia cells may play this nerve-protective role via the promotion of angiogenesis. However, the underlying mechanisms are largely unknown and need further investigation. OBJECTIVES: The aim of this study was to investigate the pro-angiogenesis effects of unpolarized, interleukin-4 (IL-4)-polarized or lipopolysaccharide (LPS)-polarized BV2 microglia cells both in vivo and in vitro. We also investigated the potential mechanisms of these pro-angiogenesis effects. MATERIAL AND METHODS: BV2 cells were polarized using phosphate-buffered saline (PBS), LPS or IL-4, respectively. The gene expression pattern was analyzed by reverse transcription polymerase chain reaction (RTPCR). The transfer of polarized BV2 cells was performed with an intravenous injection into mice 45 min after the middle cerebral artery (MCA) occlusion. Angiogenin expression was assessed by immunofluorescence. We also examined the angiogenesis effect of polarized BV2 cells and their exosomes through 3-dimensional co-cultures in vitro. Finally, the microRNA (miRNA) profiles of exosomes released by BV2 cells under different polarization conditions were examined using miRNA microarray. RESULTS: The IL-4-polarized BV2 transplantation promoted angiogenin expression in the ischemic brain. Interleukin-4-polarized microglia increased the tube formation of endothelial cells by secreting exosomes. The miRNA profiles of ex...