Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell–Cell Interactions

作者：Yun Ge, Long Chen, Shibo Liu, Jingyi Zhao, Heng Zhang, Peng R. Chen · 发表于：Journal of the American Chemical Society · 年份：2019 · DOI：10.1021/jacs.8b10286 · 被引用次数：137 · 研究领域：Biotin and Related Studies、Biochemical and Structural Characterization、Click Chemistry and Applications

Cell-cell interactions and communications play fundamental roles in life processes but remain largely uncharacterized. We developed an enzyme-mediated proximity cell labeling (EXCELL) strategy as a general method to detect and record cell-cell interactions under living conditions. EXCELL relies on an evolved Staphylococcus aureus transpeptidase sortase A variant (mgSrtA) capable of promiscuous labeling of various cell surface proteins containing a monoglycine residue at the N-terminus. Displaying mgSrtA on the surface of a cell of interest allows the labeling and detection of interacting cells in a proximity-dependent fashion.