Accelerated Wound Healing Induced by a Novel Amphibian Peptide (OA-FF10)

作者：Naixin Liu, Zhe Li, Buliang Meng, Wenxin Bian, Xiaojie Li, Siyuan Wang, Xiaoqing Cao, Yongli Song, Meifeng Yang, Ying Wang, Jing Tang, Xinwang Yang · 发表于：Protein and Peptide Letters · 年份：2019 · DOI：10.2174/0929866526666190124144027 · 被引用次数：21 · 研究领域：Antimicrobial Peptides and Activities、Silk-based biomaterials and applications、Polydiacetylene-based materials and applications

BACKGROUND: Despite the continued development of modern medicine, chronic wounds are still a critical issue in clinical treatment, placing a great physiological, psychological, and financial burden on patients. Researchers have investigated many methods to solve this problem, with bioactive peptides gaining increasing attention due to their considerable advantages and diverse functions, as well as low cost, simple storage, and easy transportation. METHODS: In this research, a novel peptide (named OA-FF10) was identified from the skin secretions of the odorous frog species Odorrana andersonii. The sequence of mature OA-FF10 was "FFTTSCRSGC", which was produced by the post-translational processing of a 61-residue prepropeptide. RESULTS: Similar to most frog peptides, OA-FF10 showed an intramolecular disulfide bridge at the C-terminus. OA-FF10 demonstrated no antibacterial, antioxidant, hemolytic, or acute toxic activity, but promoted wound healing and proliferation of human keratinocytes (HaCaT) both time- and dose-dependently. Furthermore, while OA-FF10 had no effect on wound healing of Human Skin Fibroblasts (HSF), it did accelerate healing in a full-thickness skin-wound mouse model. CONCLUSION: , thus providing a new candidate for the development of novel wound-healing drugs.