Changes in rhizosphere bacterial and fungal community composition with vegetation restoration in planted forests

作者：Gui Yao Liu, Li‐li Chen, Xinrong Shi, Zhiyou Yuan, Lois Y. Yuan, T. Ryan Lock, Robert L. Kallenbach · 发表于：Land Degradation and Development · 年份：2019 · DOI：10.1002/ldr.3275 · 被引用次数：85 · 研究领域：Microbial Community Ecology and Physiology、Forest Ecology and Biodiversity Studies、Mycorrhizal Fungi and Plant Interactions

Abstract Soil microbial communities affect nutrient cycling and ecosystem functioning. However, the variations in microbial diversity and community composition within degraded landscapes remain unclear. Using high‐throughput sequencing of bacterial 16S ribosomal RNA genes and internal transcribed spacer fungal sequences, we investigated the rhizosphere microbial diversity and community of coniferous Pinus tabulaeformis Carr. forests in degraded lands across a chronosequence that spanned over 60 years (10, 25, 40, and 60 years since restoration, four forest stands). We found significant differences in soil bacterial and fungal communities among stand ages. Actinobacteria , Proteobacteria , and Acidobacteria dominated the rhizosphere, whereas Basidiomycota, Ascomycota , and Zygomycota prevailed as fungal components. With stand development, bacterial diversity decreased, but fungal diversity increased. Nonmetric multidimensional scaling analysis separated bacterial community clusters well by stands. Fungal community clusters of 25‐ and 60‐year‐old stands overlapped. The dominant bacteria Acidobacteria showed the highest relative abundance at the 40‐year‐old stands. Soil microbial communities correlated significantly with the macro‐nutrients (soil organic carbon, total nitrogen, and total phosphorous). Additionally, the relative abundance of Acidobacteria at the phylum level correlated positively with soil total phosphorous; Deltaproteobacteria at the class level correlated posit...