Real-time visualization of autophagy by monitoring the fluctuation of lysosomal pH with a ratiometric two-photon fluorescent probe

作者：Peng Ning, Liling Hou, Yan Feng, Guoyong Xu, Yuyuan Bai, Haizhu Yu, Xiangming Meng · 发表于：Chemical Communications · 年份：2019 · DOI：10.1039/c8cc09517e · 被引用次数：73 · 研究领域：Porphyrin and Phthalocyanine Chemistry、Luminescence and Fluorescent Materials、Photochromic and Fluorescence Chemistry

A benzimidazole-decorated two-photon fluorescent probe (Lyso-MPCB) based on the p-methoxyphenylacetylene-substituted carbazole was developed for detecting lysosomal pH with a double-channel signal, which can be used to visualize autophagy by real-time imaging the fluctuation of the pH in the lysosomes.