Porous Se@SiO<sub>2</sub> nanospheres attenuate ischemia/reperfusion (I/R)-induced acute kidney injury (AKI) and inflammation by antioxidative stress

作者：Zhihuang Zheng, Guoying Deng, Chenyang Qi, Yu Xu, Xijian Liu, Zhonghua Zhao, Zhigang Zhang, Yuening Chu, Huijuan Wu, Jun Liu · 发表于：International Journal of Nanomedicine · 年份：2018 · DOI：10.2147/ijn.s184804 · 被引用次数：42 · 研究领域：Selenium in Biological Systems、Advanced Nanomaterials in Catalysis、Acute Kidney Injury Research

Objectives: Acute kidney injury (AKI) is a growing global health concern, and is associated with high rates of mortality and morbidity in intensive care units. Se is a trace element with antioxidant properties. This study aimed to determine whether porous [email protected] 2 nanospheres could relieve oxidative stress and inflammation in ischemia/reperfusion (I/R)-induced AKI. Methods: Male 6- to 8-week-old C57bl/6 mice were divided into four groups: sham + saline, sham + [email protected] 2 , I/R + saline, and I/R + [email protected] 2 . Mice in the I/R groups experienced 30 minutes of bilateral renal I/R to induce an AKI. Porous [email protected] 2 nanospheres (1 mg/kg) were intraperitoneally injected into mice in the I/R + [email protected] 2 group 2 hours before I/R, and the same dose was injected every 12 hours thereafter. Hypoxia/reoxygenation (H/R) was used to mimic I/R in vitro. PBS was used as a control treatment. Human kidney 2 cells were seeded into 12-well plates (5×105 cells/well) and divided into four groups: control + PBS group, control + [email protected] 2 group, H/R + PBS group, and H/R + [email protected] 2 group (n=3 wells). We then determined the expression levels of ROS, glutathione, inflammatory cytokines and proteins, fibrosis proteins, and carried out histological analysis upon kidney tissues. Results: In vitro, intervention with porous [email protected] 2 nanospheres significantly reduced levels of ROS ( P <0.05), inflammatory cytokines ( P <0.05), an...