Modulation of the Gut Microbiota in Rats by Hugan Qingzhi Tablets during the Treatment of High‐Fat‐Diet‐Induced Nonalcoholic Fatty Liver Disease

作者：Waijiao Tang, Xiaorui Yao, Fan Xia, Miaoting Yang, Zhijuan Chen, Benjie Zhou, Qiang Liu · 发表于：Oxidative Medicine and Cellular Longevity · 年份：2018 · DOI：10.1155/2018/7261619 · 被引用次数：162 · 研究领域：Liver Disease Diagnosis and Treatment、Gut microbiota and health、Metabolomics and Mass Spectrometry Studies

Background . Accumulative evidence showed that gut microbiota was important in regulating the development of nonalcoholic fatty liver disease (NAFLD). Hugan Qingzhi tablet (HQT), a lipid‐lowering and anti‐inflammatory medicinal formula, has been used to prevent and treat NAFLD. However, its mechanism of action is unknown. The aim of this study was to confirm whether HQT reversed the gut microbiota dysbiosis in NAFLD rats. Methods . We established an NAFLD model of rats fed with a high‐fat diet (HFD), which was given different interventions, and measured the level of liver biochemical indices and inflammatory factors. Liver tissues were stained with hematoxylin‐eosin and oil red O. Changes in the gut microbiota composition were analyzed using 16S rRNA sequencing. Results . The hepatic histology and biochemical data displayed that HQT exhibited protective effects on HFD‐induced rats. Moreover, HQT also reduced the abundance of the Firmicutes / Bacteroidetes ratio in HFD‐fed rats and modified the gut microbial species at the genus level, increasing the abundances of gut microbiota which were reported to have an effect on relieving NAFLD, such as Ruminococcaceae , Bacteroidales_S24-7_group , Bifidobacteria , Alistipes , and Anaeroplasma , and significantly inhibiting the relative abundance of Enterobacteriacea e, Streptococcus , Holdemanella , Allobaculum , and Blautia , which were reported to be potentially related to NAFLD. Spearman’s correlation analysis found that [Ruminococc...