Expressions of miR‐525‐3p and its target gene SEMG 1 in the spermatozoa of patients with asthenozoospermia

作者：Qidong Zhou, Xiaobin Guo, W.‐s. Zhang, Jiaying Zhou, Cheng Yang, Jun Bian, Mingkun Chen, Wenbin Guo, P. Wang, Tiewei Qi, Chenqiu Wang, Jing Yang, Cundong Liu · 发表于：Andrology · 年份：2018 · DOI：10.1111/andr.12573 · 被引用次数：56 · 研究领域：Sperm and Testicular Function、Reproductive biology and impacts on aquatic species、Ovarian function and disorders

BACKGROUND: Semenogelin 1 (SEMG1) is an important secretory protein in spermatozoa involved in the formation of a gel matrix encasing ejaculated spermatozoa. Previous studies show that the SEMG1 gene is highly expressed in spermatozoa from patients with asthenozoospermia (AZS); however, the underlying molecular mechanisms are not yet clear. OBJECTIVES: To study the molecular mechanism of high expression of SEMG1 gene and its potential roles in AZS. MATERIALS AND METHODS: Western blot and real-time PCR were used to detect the expression levels of SEMG1 protein and mRNA in the ejaculated spermatozoa from normozoospermic males and AZS patients. Bioinformatics analysis was used to predict miRNAs targeting for SEMG1 3'-untranslated region detection of the expression levels of all the candidate miRNAs in ejaculatory spermatozoa in AZS patients or normozoospermic volunteers. Luciferase reporter assays were performed to confirm it can directly bind to SEMG1. Correlation of miR-525-3p and SEMG1 mRNA expression with clinical sperm parameters were also analyzed. Finally, we conducted a follow-up study of reproductive history about all the subjects. RESULTS: SEMG1 mRNA and protein level were significantly higher in AZS patients compared to that in normozoospermic volunteers (p < 0.001). Subsequently, microRNA-525-3p (miR-525-3p) which was predicted as a candidate regulator of SEMG1 was found lower expressed in ejaculatory spermatozoa in AZS patients (p = 0.0074). Luciferase experiment re...