F-box protein RAE1 regulates the stability of the aluminum-resistance transcription factor STOP1 in Arabidopsis

作者：Yang Zhang, Jie Zhang, Jinliang Guo, Fanglin Zhou, Somesh Singh, Xuan Xu, Qi Xie, Zhong‐Bao Yang, Chao‐Feng Huang · 发表于：Proceedings of the National Academy of Sciences · 年份：2018 · DOI：10.1073/pnas.1814426116 · 被引用次数：153 · 研究领域：Aluminum toxicity and tolerance in plants and animals、Silicon Effects in Agriculture、Plant Stress Responses and Tolerance

Aluminum (Al) toxicity is a major factor limiting crop production on acid soils, which represent over 30% of the world’s arable land. Some plants have evolved mechanisms to detoxify Al. Arabidopsis , for example, secretes malate via the AtALMT1 transporter to chelate and detoxify Al. The C2H2-type transcription factor STOP1 plays a crucial role in Al resistance by inducing the expression of a set of genes, including AtALMT1 . Here, we identify and characterize an F-box protein-encoding gene regulation of Atalmt1 expression 1 ( RAE1 ) that regulates the level of STOP1. Mutation and overexpression of RAE1 increases or decreases the expression of AtALMT1 and other STOP1-regulated genes, respectively. RAE1 interacts with and promotes the degradation of STOP1 via the ubiquitin-26S proteasome pathway, while Al stress promotes the accumulation of STOP1. We find that STOP1 up-regulates RAE1 expression by directly binding to the RAE1 promoter, thus forming a negative feedback loop between STOP1 and RAE1. Our results demonstrate that RAE1 influences Al resistance through the ubiquitination and degradation of STOP1.