A novel on‐chip solution enabling rapid analysis of melamine and chloramphenicol in milk by smartphones

作者：Ning Yang, Liangliang Xie, Chen Pan, Minfeng Yuan, Zhi‐Han Tao, Han‐Ping Mao · 发表于：Journal of Food Process Engineering · 年份：2018 · DOI：10.1111/jfpe.12976 · 被引用次数：31 · 研究领域：Biosensors and Analytical Detection、Advanced biosensing and bioanalysis techniques、Melamine detection and toxicity

Abstract Ubiquitous food security control is desirable. However, the analysis methods, such as those for milk, are highly based on manual analysis, leading to low efficiency. Thus, we report a multilayer paper‐based microfluidic chip transforming chromatic readout to electric signals. First, colloidal gold immunochromatographic assays (GICA) are used to achieve the colorimetric occurrence. Secondly, the test (T) lines and control (C) lines are patterned to encode different EAN‐8 barcode. Furthermore, accurate scanning is accomplished by the automatic threshold segmentation. These technologies make the digital determination of melamine (MEL) and chloramphenicol (CAP) by phone readily accessible. Results indicate that the accurate machine‐scanning can be assured when MEL and CAP is above 30 ng/mL and 6 ng/mL in milk respectively. The detection time is under 8 min, and the stable storage life is over 90 days. Therefore, this simple chip allows rapid, machine‐readable, electric‐recordable diagnostic directly from chromatic assays with satisfying stability, specificity and accuracy. Practical applications This article illustrates a method used to determine the concentration of melamine and chloramphenicol in milk. When melamine and chloramphenicol is above 30 and 6 ng/ml in milk, the produced EAN‐8 barcode can be recognized with high accurate level, compared with international standards, the results are acceptable and sensitive. This method is highly intellectual and environmental...