New Rice-Derived Short Peptide Potently Alleviated Hyperuricemia Induced by Potassium Oxonate in Rats

作者：Naixin Liu, Ying Wang, Meifeng Yang, Wenxin Bian, Lingling Zeng, Saige Yin, Ziqian Xiong, Yan Hu, Siyuan Wang, Buliang Meng, Jun Sun, Xinwang Yang · 发表于：Journal of Agricultural and Food Chemistry · 年份：2018 · DOI：10.1021/acs.jafc.8b05879 · 被引用次数：83 · 研究领域：Gout, Hyperuricemia, Uric Acid、Pharmacological Effects of Natural Compounds、Exercise and Physiological Responses

Gout that caused by hyperuricemia affects human health seriously and more efficient drugs are urgently required clinically. In this study, a novel peptide named RDP1 (AAAAGAKAR, 785.91 Da) was identified from the extract of shelled fruits of Oryza sativa. Our results demonstrated that RDP1 (the minimum effective concentration is 10 μg/kg) could significantly reduce the serum uric acid and creatinine and alleviate hyperuricemic nephropathy in rats by intragastric administration. RDP1 inhibited xanthine oxidase, which also was verified at the animal level. Results from molecular docking indicated that RDP1 can inhibit uric acid formation by occupying the binding site of xanthine oxidase to xanthine. Besides, RDP1 showed no toxicity on rats and was stable in several temperatures, demonstrating its advantages for transportation. This research was the first discovery of antihyperuricemic peptide from the shelled fruits of O. Sativa and provided a new candidate for the development of hypouricemic drugs.