Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment

作者：Dingyu Wu, Zhenxing Wang, Jinbing Wang, Yingnan Geng, Zhanzhao Zhang, Zhanzhao Zhang, Yu Li, Qiannan Li, Zhiwei Zheng, Yilin Cao, Zhiyong Zhang, Zhiyong Zhang · 发表于：Stem Cell Research & Therapy · 年份：2018 · DOI：10.1186/s13287-018-1064-1 · 被引用次数：40 · 研究领域：Bone Tissue Engineering Materials、3D Printing in Biomedical Research、Tissue Engineering and Regenerative Medicine

BACKGROUND: Bone tissue engineering is not widely used in clinical treatment. Two main reasons hide behind this: (1) the seed cells are difficult to obtain and (2) the process of tissue engineering bone construction is too complex and its efficiency is still relatively low. It is foreseeable that in the near future, the problem of seed cell sources could be solved completely in tissue engineering bone repair. As for the complex process and low efficiency of tissue engineering bone construction, usually two strategies would be considered: (1) the construction strategy based on injectable bone tissue and (2) the construction strategy based on osteogenic cell sheets. However, the application of injectable bone tissue engineering (iBTE) strategy and osteogenic cell sheet strategy is limited and they could hardly be used directly in repairing defects of large segmental bone, especially load-bearing bone. METHODS: In this study, we built an osteogenic micro-tissue with simple construction but with a certain structure and composition. Based on this, we established a new iBTE repair strategy-osteogenic micro-tissue in situ repair strategy, mainly targeting at solving the problem of large segmental bone defect. The steps are as follows: (1) Build the biodegradable three-dimensional scaffold based on the size of the defect site with 3D printing rapid prototyping technology. (2) Implant the three-dimensional scaffold into the defect site. This scaffold is considered as the "steel framew...