Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo

作者：Muhammad Imran Khan, Abdulatef Mrghni Ahhmed, Jin Hyuk Shin, Jun Soo Baek, Min Yong Kim, Jong Deog Kim · 发表于：Evidence-based Complementary and Alternative Medicine · 年份：2018 · DOI：10.1155/2018/3486106 · 被引用次数：110 · 研究领域：Tea Polyphenols and Effects、Natural product bioactivities and synthesis、Traditional Chinese Medicine Analysis

Bacteria are one of the major causes of severe infections and diseases of plants and animals. Salmonella are crucially important due to infection in poultry leading to huge economical loses. Due to high cost and microbial resistance to the currently available chemical antibiotics, demand of screening natural products with antibiotics effects is increased. Plants are rich sources of natural bioactive compounds with antibiotic effects. Saponins are natural compounds of plant sources having a diverse range of applications. In present study we investigated the in vitro and in vivo antibacterial activities of green tea seed extracted saponins. Green tea seeds crude extract was prepared in 70% ethanol by continuous reflux in heating mantel for 5 hours. Crude saponins were extracted from the crude ethanolic extract of green tea seed by column chromatography using macroporous resin (D101). Saponin mixture in fraction 1 (Fr1) was obtained from crude saponins extract via column chromatography. Fr2 and Fr3 were isolated from saponins mixture by preparative HPLC. Antibacterial activities of the isolated saponins fractions were investigated against Escherichia coli (ATCC 25922), Streptococcus aureus (ATCC 12600), and six serovars of Salmonella. In vitro antibacterial activities were determined by disc‐diffusion method and growth inhibition in liquid culture using 96‐well plate. Results showed that the green tea isolated saponins fractions possess antibacterial effects in the following ord...