Total Internal Reflection‐Based Extinction Spectroscopy of Single Nanoparticles

作者：Meng Li, Tinglian Yuan, Ying‐Yan Jiang, Linlin Sun, 韦玮 Wei Wei, Hong‐Yuan Chen, Wei Wang · 发表于：Angewandte Chemie International Edition · 年份：2018 · DOI：10.1002/anie.201810324 · 被引用次数：33 · 研究领域：Gold and Silver Nanoparticles Synthesis and Applications、Advanced Fluorescence Microscopy Techniques、Quantum Dots Synthesis And Properties

Abstract Herein we report a reflection‐mode total internal reflection microscopy (TIRM) to measure the extinction spectrum of individual dielectric, plasmonic, or light‐absorbing nanoparticles, and to differentiate absorption and scattering components from the total optical output. These capabilities were enabled via illuminating the sample with evanescent wave of which the lightpath length was comparable with the size of single nanoparticles, leading to a dramatically improved reflectance change (Δ I / I 0 ) up to tens of percent. It was further found that scattering and absorption of light contributed to bright and dark centroids, respectively, in the optical patterns of single nanoparticles, allowing to distinguish scattering and absorption components from the extinction spectrum by the use of an appropriate image processing method. In addition, wide‐field feature of TIRM enabled the studies on tens of nanoparticles simultaneously with gentle illumination.