Regeneration of a Bioengineered Thyroid Using Decellularized Thyroid Matrix

作者：Jun Pan, Hui Li, Fang Yun, Yi-Bin Shen, Xueyu Zhou, Feng Zhu, Li-Xian Zhu, Yehui Du, Xiongfei Yu, Yan Wang, Yan Wang, Xinhui Zhou, Yingying Wang, Yingying Wang, Yi‐Jun Wu · 发表于：Thyroid · 年份：2018 · DOI：10.1089/thy.2018.0068 · 被引用次数：21 · 研究领域：Tissue Engineering and Regenerative Medicine、Bone Tissue Engineering Materials、Xenotransplantation and immune response

BACKGROUND: Hypothyroidism is a common hormone deficiency condition. Regenerative medicine approaches, such as a bioengineered thyroid, have been proposed as potential therapeutic alternatives for patients with hypothyroidism. This study demonstrates a novel approach to generate thyroid grafts using decellularized rat thyroid matrix. METHODS: Isolated rat thyroid glands were perfused with 1% sodium dodecyl sulfate to generate a decellularized thyroid scaffold. The rat thyroid scaffold was then recellularized with rat thyroid cell line to reconstruct the thyroid by perfusion seeding technique. As a pilot study, the decellularized rat thyroid scaffold was perfused with human-derived thyrocytes and parathyroid cells. RESULTS: The decellularization process retained the intricate three-dimensional microarchitecture with a perfusable vascular network and native extracellular matrix components, allowing efficient reseeding of the thyroid matrix with the FRTL-5 rat thyroid cell line generating three-dimensional follicular structures in vitro. In addition, the recellularized thyroid showed successful cellular engraftment and thyroid-specific function, including synthesis of thyroglobulin and thyroid peroxidase. Moreover, the decellularized rat thyroid scaffold could further be recellularized with human-derived thyroid cells and parathyroid cells to reconstruct a humanized bioartificial endocrine organ, which maintained expression of critical genes such as thyroglobulin, thyroid peroxi...