The Simplified Carbapenem Inactivation Method (sCIM) for Simple and Accurate Detection of Carbapenemase-Producing Gram-Negative Bacilli

作者：Xiaopeng Jing, Huan Zhou, Xiaochun Min, Xing Zhang, Qing Yang, Shuaixian Du, Yirong Li, Fangyou Yu, Min Jia, Yu Zhan, Yi Zeng, Bo Yang, Yunjun Pan, Binghuai Lu, Rong Liu, Ji Zeng · 发表于：Frontiers in Microbiology · 年份：2018 · DOI：10.3389/fmicb.2018.02391 · 被引用次数：74 · 研究领域：Antibiotic Resistance in Bacteria、Vibrio bacteria research studies、Pharmaceutical and Antibiotic Environmental Impacts

This study reportedaimed to design a method, called the simplified carbapenem inactivation method (sCIM), for the simple and accurate detection of carbapenemase-producing gram-negative bacilli. This method is based on modified carbapenem inactivation method (mCIM) with improvement of experimental procedures: Instead of incubating the antibiotic disk in the organism culture media, organism to be test was smeared directly onto antibiotic disk. for simple and accurate detection of carbapenemase phenotypes. ForWe evaluatinged the sensitivity and specificity of the method, called the simplified carbapenem inactivation method (sCIM), for the detection of carbapenemase-producing gram-negative bacilli. A a total of 196 Enterobacteriaceae, 73 Acinetobacter baumannii, and 158 Pseudomonas aeruginosa isolates were collected. PCR was used to detect carbapenemase genes. Phenotypic evaluations were performed using both the sCIM and mCIM. PCR results showed that, of the 196 Enterobacteriaceae strains, 147 expressed the carbapenemase genes blaKPC-2 (58.5%), blaIMP-4 (21.8%), blaIMP-2 (2.0%), blaVIM-1 (6.1%), blaNDM-1 (10.2%) and blaOXA-48 (1.4%). sCIM results had high concordance with PCR results (99.5%) and mCIM results (100%) with the exception of one Klebsiella pneumoniae strain, which had an MIC for imipenem of 0.25 mg/L. PCR demonstrated that 53 of 73 A. baumannii isolates expressed the carbapenemase genes blaOXA-23 (98.1%) and blaVIM-2 (1.8%). sCIM and PCR results corresponded but all A...