Jejunal Metabolic Responses to Escherichia coli Infection in Piglets

作者：Hucong Wu, Jiaqi Liu, Siyuan Chen, Yuanyuan Zhao, Sijing Zeng, Peng Bin, Dong Zhang, Zhiyi Tang, Guoqiang Zhu · 发表于：Frontiers in Microbiology · 年份：2018 · DOI：10.3389/fmicb.2018.02465 · 被引用次数：15 · 研究领域：Diet and metabolism studies、Clinical Nutrition and Gastroenterology、Pharmacological Effects of Natural Compounds

CFUs (colony-forming units) of ETEC W25K and assigned into diarrheal, recovered, control, and resistant groups. Jejunal samples were harvested at day 6 and metabolic profiles were analyzed via gas chromatography coupled to time-of-flight mass spectrometry (GC/TOFMS). The results showed that 33 metabolites in the jejunum were identified in ETEC-induced diarrhea, including amino acids, fatty acids, sugars, and organic acids. Compared with the control, resistant, and recovered piglets, diarrheal piglets showed higher concentrations of 4-aminobutyric acid (GABA) and glycine in the jejunum. Compared with the control and resistant piglets, six metabolites were markedly decreased in diarrheal piglets, including ornithine, asparagine, glutamine, citric acid, citrulline, and lysine. Collectively, this study provides insights into jejunal metabolic response to ETEC infection and ETEC induced diarrhea in piglets.