Comparative Study on Alternative Splicing in Human Fungal Pathogens Suggests Its Involvement During Host Invasion

作者：Patricia Sieber, Kerstin Voigt, Philipp Kämmer, Sascha Brunke, Stefan Schuster, Jörg Linde · 发表于：Frontiers in Microbiology · 年份：2018 · DOI：10.3389/fmicb.2018.02313 · 被引用次数：68 · 研究领域：Fungal Infections and Studies、Plant Disease Resistance and Genetics、Antifungal resistance and susceptibility

Alternative splicing (AS) is an important regulatory mechanism in eukaryotes but only little is known about its impact in fungi. Human fungal pathogens are of high clinical interest causing recurrent or life-threatening infections. AS can be well-investigated genome-wide and quantitatively with the powerful technology of RNA-Seq. Here, we systematically studied AS in human fungal pathogens based on RNA-Seq data. To do so, we investigated its effect in seven fungi during conditions simulating \textit{ex vivo} infection processes and during \textit{in vitro} stress. Genes undergoing AS are species-specific and act independently from differentially expressed genes pointing to an independent mechanism to change abundance and functionality. \textit{Candida} species stand out with a low number of introns with higher and more varying lengths and more alternative splice sites. Moreover, we identified a functional difference between infection-related and other stress conditions: During stress, AS affects more genes and is involved in diverse regulatory functions. In contrast, during infection-related conditions, genes undergoing AS have membrane functionalities and might be involved in the interaction with the host. We assume that AS plays a crucial regulatory role in pathogenic fungi and is important in response to both infection-related and stress conditions.