Inhibitive Effects of FGF2/FGFR1 Pathway on Astrocyte-Mediated Inflammation in vivo and in vitro After Infrasound Exposure

作者：Yajun Shi, Ming Shi, Li-Jun Xiao, Li Li, Lin-Hui Zou, Chaoyang Li, Qin-Jun Zhang, Linfu Zhou, Xinchao Ji, Huan Huang, Xi Ye, Ling Liu, Hongya Zhang, Gang Zhao, Lei Ma · 发表于：Frontiers in Neuroscience · 年份：2018 · DOI：10.3389/fnins.2018.00582 · 被引用次数：29 · 研究领域：Noise Effects and Management、Photoacoustic and Ultrasonic Imaging、Spaceflight effects on biology

Infrasound, a kind of ambient noise, can cause severely disorders to various human organs, specially to central nervous system (CNS). Our previous studies have proved that infrasound-induced CNS injury was closely related with astrocytes activation and astrocytes-mediated neuroinflammation, but the underlying molecular mechanisms are still largely unclear. FGF2/FGFR1 (Fibroblast growth factor 2/Fibroblast growth factor receptor 1) pathway was reported to play an important role in anti-inflammation in CNS disorders. To further study the possible roles of FGF2/FGFR1 pathway in infrasound-induced CNS injury, here we exposed Sprague-Dawley rats or cultured astrocytes to 16Hz, 150dB infrasound, and explored the effects of FGF2 on infrasound-induced astrocytes activation and neuroinflammation. Western blotting, immunofluorescence and liquid chip method were used in this experiment. Our results showed that after 3-, or 7-day exposure (2h/day) of rats as well as 2h exposure of cultured astrocytes to 16Hz, 150dB infrasound, astrocyte-expressed FGFR1 was downregulated in vivo and in vitro. FGF2 pretreatment not only inhibited infrasound-induced astrocyte activation in rat hippocampal CA1 region, but also reduced the levels of pro-inflammatory cytokines, such as TNF-α, IL-1β, IL-18, IL-6 and IFN-γ in vitro and in vivo. However, FGF2 significantly upregulated the expression of FGFR1. Furthermore, we showed that FGF2 could attenuate IκBα phosphorylation, NF-κB p65 translocation, pro-infla...