A Genome-Wide Association Study Reveals a Rich Genetic Architecture of Flour Color-Related Traits in Bread Wheat

作者：Shengnan Zhai, Jindong Liu, Dengan Xu, Weie Wen, Jun Yan, Pingzhi Zhang, Yingxiu Wan, Shuanghe Cao, Yuanfeng Hao, Xianchun Xia, Wujun Ma, Zhonghu He · 发表于：Frontiers in Plant Science · 年份：2018 · DOI：10.3389/fpls.2018.01136 · 被引用次数：40 · 研究领域：Wheat and Barley Genetics and Pathology、Genetic Mapping and Diversity in Plants and Animals、Horticultural and Viticultural Research

Flour color-related traits, including brightness (L*), redness (a*), yellowness (b*) and yellow pigment content (YPC), are very important for end-use quality of wheat. Uncovering the genetic architecture of these traits is necessary for improving wheat quality by marker-assisted selection (MAS). In the present study, a genome-wide association study (GWAS) was performed on a collection of 166 bread wheat cultivars to better understand the genetic architecture of flour color-related traits using the wheat 90K and 660K SNP arrays, and ten allele-specific markers for known genes influencing these traits. Fifteen, 28, 25 and 32 marker–trait associations (MTAs) for L*, a*, b* and YPC, respectively, were detected, explaining 6.5–20.9% phenotypic variation. Seventy-eight loci were consistent across all four environments. Compared with previous studies, Psy-A1, Psy-B1, Pinb-D1 and the 1B•1R translocation controlling flour color-related traits were confirmed, and four loci were novel. Two and 11 loci explained much more phenotypic variation of a* and YPC than phytoene synthase 1 gene (Psy1), respectively. Sixteen candidate genes were predicted based on biochemical information and bioinformatics analyses, mainly related to carotenoid biosynthesis and degradation, terpenoid backbone biosynthesis and glycolysis/gluconeogenesis. The results largely enrich our knowledge of the genetic basis of flour color-related traits in bread wheat and provide valuable markers for wheat quality improveme...