Comprehensive analysis of immune evasion in breast cancer by single-cell RNA-seq

作者：Jianhua Yin, Zhisheng Li, Chen Yan, Enhao Fang, Ting Wang, Hanlin Zhou, Weiwei Luo, Qing Zhou, Jingyu Zhang, Jintao Hu, Haoxuan Jin, Lei Wang, Xing Zhao, Jiguang Li, Xiaojuan Qi, Wenbin Zhou, Chen Huang, Chenyang He, Huanming Yang, Karsten Kristiansen, Yong Hou, Shida Zhu, Dongxian Zhou, Ling Wang, Michael Dean, Kui Wu, Hong Hu, Guibo Li · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2018 · DOI：10.1101/368605 · 被引用次数：8 · 研究领域：Single-cell and spatial transcriptomics、Immune cells in cancer、Cancer Immunotherapy and Biomarkers

Abstract The tumor microenvironment is composed of numerous cell types, including tumor, immune and stromal cells. Cancer cells interact with the tumor microenvironment to suppress anticancer immunity. In this study, we molecularly dissected the tumor microenvironment of breast cancer by single-cell RNA-seq. We profiled the breast cancer tumor microenvironment by analyzing the single-cell transcriptomes of 52,163 cells from the tumor tissues of 15 breast cancer patients. The tumor cells and immune cells from individual patients were analyzed simultaneously at the single-cell level. This study explores the diversity of the cell types in the tumor microenvironment and provides information on the mechanisms of escape from clearance by immune cells in breast cancer. One Sentence Summary Landscape of tumor cells and immune cells in breast cancer by single cell RNA-seq