A Scalable “Junction Substrate” to Engineer Robust DNA Circuits

作者：Xianbao Sun, Wei Bing, Yijun Guo, Shiyan Xiao, Xiang Li, Dongbao Yao, Xue Yin, Shiyong Liu, Haojun Liang · 发表于：Journal of the American Chemical Society · 年份：2018 · DOI：10.1021/jacs.8b05203 · 被引用次数：42 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA Interference and Gene Delivery、DNA and Nucleic Acid Chemistry

Versatile building blocks are essential for building complex and scaled-up DNA circuits. In this study, we propose a conceptually new scalable architecture called a "junction substrate" (J-substrate) that is linked by prepurified double-stranded DNA molecules. As a proof-of-concept, this novel type of substrate has been utilized to build multi-input DNA circuits, offering several advantages over the conventional substrate (referred to as a "linear substrate", L-substrate). First, the J-substrate does not require long DNA strands, thus avoiding significant synthetic errors and costs. Second, the traditional PAGE purification method is technically facilitated to obtain high-purity substrates, whereby the initial leakage is effectively eliminated. Third, the asymptotic leakage is eliminated by introducing the "junction". Finally, circuits with the optimized J-substrate architecture exhibit fast kinetics. We believe that the proposed architecture constitutes a sophisticated chassis for constructing complex circuits.