Nanostructured titanium regulates osseointegration via influencing macrophage polarization in the osteogenic environment

作者：Jinjin Wang, Fanhui Meng, Wen Song, Jingyi Jin, Qianli Ma, Dongdong Fei, Liang Fang, Lihua Chen, Qintao Wang, Yumei Zhang · 发表于：International Journal of Nanomedicine · 年份：2018 · DOI：10.2147/ijn.s163956 · 被引用次数：136 · 研究领域：Bone Tissue Engineering Materials、Bone Metabolism and Diseases、Graphene and Nanomaterials Applications

Introduction: Fabricating nanostructured surface topography represents the mainstream approach to induce osteogenesis for the next-generation bone implant. In the past, the bone implant was designed to minimize host repulsive reactions in order to acquire biocompatibility. However, increasing reports indicate that the absence of an appropriate immune response cannot acquire adequate osseointegration after implantation in vivo. Materials and methods: We prepared different topographies on the surface of titanium (Ti) specimens by grinding, etching and anodizing, and they were marked as polished specimen (P), specimen with nanotubes (NTs) in small diameters (NT-30) and specimen with NTs in large diameters (NT-100). We evaluated the ability of different topographies of the specimen to induce osteogenic differentiation of mice bone marrow mesenchymal stem cells (BMSCs) in vitro and to induce osseointegration in vivo. Furthermore, we investigated the effect of different topographies on the polarization and secretion of macrophages, and the effect of macrophage polarization on topography-induced osteogenic differentiation of mice BMSCs. Finally, we verified the effect of macrophage polarization on topography-induced osseointegration in vivo by using Cre*RBP-J fl/fl mice in which classically activated macrophage was restrained. Results: The osteogenic differentiation of mice BMSCs induced by specimen with different topographies was NT-100>NT-30>P, while the osseointegration induced b...