Screening for biomarkers reflecting the progression of Babesia microti infection

作者：Bin Xu, Xiufeng Liu, Yu-Chun Cai, Ji-Lei Huang, Rui‐xiang Zhang, Jun‐Hu Chen, Xunjia Cheng, Xia Zhou, Xue-Nian Xu, Yan Zhou, Ting Zhang, Shen‐Bo Chen, Jian Jian Li, Qunfeng Wu, Chengsong Sun, Yongfeng Fu, Jiaxu Chen, Xiao‐Nong Zhou, Wei Hu · 发表于：Parasites & Vectors · 年份：2018 · DOI：10.1186/s13071-018-2951-0 · 被引用次数：33 · 研究领域：Vector-borne infectious diseases、Parasites and Host Interactions、Invertebrate Immune Response Mechanisms

BACKGROUND: Babesiosis is caused by the invasion of erythrocytes by parasites of the Babesia spp. Babesia microti is one of the primary causative agents of human babesiosis. To better understand the status of the disease, discovering key biomarkers of the different infection stages is crucial. RESULTS: This study investigated B. microti infection in the mouse model from 0 to 270 days post-infection (dpi), using blood smears, PCR assays and ELISA. PCR assays showed a higher sensitivity when compared to microscopic examination. Specific IgG antibodies could be detected from 7 days to 270 dpi. Two-dimensional electrophoresis was combined with western blotting and mass spectrometric analysis to screen for specific reactive antigens during both the peak parasitaemia period (7 dpi) and IgG antibody response peak period (30 dpi) by the infected mice plasma. The 87 positive reactive proteins were identified and then expressed with the wheat germ cell-free system. Protein microarrays of all 87 targeted proteins were produced and hybridized with the serial plasma of infected mice model. Based on the antigen reaction profile during the infection procedure, 6 antigens were selected and expressed in Escherichia coli. Due to an early response to IgM, lower immunoreactivity levels of IgG after two months and higher immunoreactivity level IgG during nine months, four recombinant proteins were selected for further characterization, namely rBm2D97(CCF75281.1), rBm2D33(CCF74637.1), rBm2D41(CCF7...