Anti‑inflammatory action of ambuic acid, a natural product isolated from the solid culture of Pestalotiopsis�neglecta, through blocking ERK/JNK mitogen‑activated protein kinase signaling pathway

作者：Qian Zhang, Ruiling Luan, Huixiang Li, Yanan Liu, Pan Liu, Liying Wang, Danna Li, Mengdi Wang, Qiang Zou, Hongwei Liu, Keiichi Matsuzaki, Feng Zhao · 发表于：Experimental and Therapeutic Medicine · 年份：2018 · DOI：10.3892/etm.2018.6294 · 被引用次数：22 · 研究领域：Microbial Natural Products and Biosynthesis、Fungal Biology and Applications、Plant chemical constituents analysis

Ambuic acid is an organic acid isolated from the solid culture of Pestalotiopsis neglecta, which is an endophytic fungus that widely exists in many species of plants. Ambuic acid has been reported to exert antimicrobial activity against Gram‑positive bacterium. The aim of the present study was to investigate the inhibitory effect of ambuic acid on lipopolysaccharide (LPS)‑induced inflammation in RAW264.7 macrophages. The results demonstrated that ambuic acid significantly suppressed the overproduction of nitric oxide (NO) and prostaglandin E2 (PGE2) in a dose‑dependent manner. Furthermore, ambuic acid also inhibited the release of the proinflammatory cytokine interleukin‑6 (IL‑6) however, no inhibition of the release of tumor necrosis factor‑α (TNF‑α) was observed. Further investigations indicated that ambuic acid downregulated the LPS‑induced high expression of inducible NO synthase (iNOS) and cyclooxygenase‑2 (COX‑2) proteins, as well as inhibited the enzymatic activity of iNOS and COX‑2. In addition, ambuic acid suppressed the phosphorylation of extracellular signal‑regulated kinase 1/2 (ERK 1/2) and c‑Jun N‑terminal kinase (JNK) induced by LPS. However, ambuic acid did not inhibit the phosphorylation of p38 mitogen‑activated protein kinase (MAPK), the degradation of IκB‑α protein or the nuclear translocation of nuclear transcription factor‑κB (NF‑κB) p65 subunit. These results suggested that ambuic acid may exert anti‑inflammatory action by blocking the activation of the ...