Wheat CBL-interacting protein kinase 23 positively regulates drought stress and ABA responses

作者：Xiaoyu Cui, Yong-Tao Du, Jin‐Dong Fu, Tai‐Fei Yu, Chang-Tao Wang, Ming Chen, Jun Chen, You‐Zhi Ma, Zhao‐Shi Xu · 发表于：BMC Plant Biology · 年份：2018 · DOI：10.1186/s12870-018-1306-5 · 被引用次数：128 · 研究领域：Plant Stress Responses and Tolerance、Plant-Microbe Interactions and Immunity、Aluminum toxicity and tolerance in plants and animals

BACKGROUND: The calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) signaling pathway responds to various abiotic stresses in plants. RESULTS: Wheat CIPK23, isolated from wheat drought transcriptome data set, was induced by multiple abiotic stresses, including drought, salt, and abscisic acid (ABA). Compared with wild-type plants, TaCIPK23-overexpression wheat and Arabidopsis showed an higher survival rate under drought conditions with enhanced germination rate, developed root system, increased accumulation of osmolytes, and reduced water loss rate. Over-expression of TaCIPK23 rendered transgenic plants ABA sensitivity, as evidenced by delayed seed germination and the induction of stomatal closure. Consistent with the ABA-sensitive phenotype, the expression level of drought- and ABA-responsive genes were increased under drought conditions in the transgenic plants. In addition, using yeast two-hybrid system, pull-down and bimolecular fluorescence complementation (BiFc) assays, TaCIPK23 was found to interact with TaCBL1 on the plasma membrane. CONCLUSIONS: These results suggest that TaCIPK23 plays important roles in ABA and drought stress responses, and mediates crosstalk between the ABA signaling pathway and drought stress responses in wheat.