Determination of progesterone concentration during the estrous cycle in dairy cows using a chemiluminescence assay

作者：Luiz Ernandes Kozicki, H. Wéber, Louise Helene Bacher, Isabella Sellmer Ramos, Francisco Romano Gaievski, Marcos Schaus, Rafaela Talini, Romildo Romualdo Weiss, Tácia Gomes Bergstein‐Galan, Márcio Sporski Segui, Carlos Alberto Mayora Aita · 发表于：Revista Acadêmica Ciência Animal · 年份：2018 · DOI：10.7213/1981-4178.2018.16006 · 被引用次数：8 · 研究领域：Reproductive Physiology in Livestock、Animal Behavior and Welfare Studies

The study aimed to determine the patterns of serum progesterone concentration in estrous cycle in dairy cows by a chemiluminescence assay (CLIA). Four non-lactating multiparous Jersey cows were used. Animals with a corpus luteum (CL) in any of the ovaries were induced into estrus. Day zero (d0) of the estrous cycle was defined as the day of visible estrus. Blood samples were collected and ultrasonography (US) of the ovaries were performed until a new manifestation of visible estrus was observed. The lengths of the estrous cycles (estrus to estrus) of the four cows were 20, 21, 22, and 23 days. The mean serum concentrations of P4 (x ± s) were 2.8 ± 1.4 ng/mL in proestrus, 2.4 ± 1.5 ng/mL in estrus, 2.0 ± 1.8 ng/mL in metestrus, and 11.9 ± 5.7 ng/mL in diestrus. The follicular and luteal phases of the estrous cycle were established based on P4 concentrations. P4 serum concentrations ≥5.48 ng/mL indicated the presence of functional CL, which was observed from d3 to d12 of the cycle. P4 concentrations decreased from d13 until next estrus. Thus, the previously mentioned P4 serum concentration was established as the limit for a predominantly functional CL. P4 concentrations < 5.48 ng/mL indicated a nonfunctional CL or CL that is not yet fully formed, which is observed in metestrus. A P4 standard curve, constructed based on the Lorentz distribution, was used to determine values of < 5.48 ng/mL of serum P4 concentration for the follicular phase and ≥ 5.48 ng/mL for the luteal p...