Structural basis of the lipid transfer mechanism of phospholipid transfer protein (PLTP)

作者：Meng Zhang, Xiaobo Zhai, Jinping Li, John J. Albers, Simona Vuletic, Gang Ren · 发表于：Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · 年份：2018 · DOI：10.1016/j.bbalip.2018.06.001 · 被引用次数：28 · 研究领域：Lipid Membrane Structure and Behavior、Lipoproteins and Cardiovascular Health、RNA and protein synthesis mechanisms

Human phospholipid transfer protein (PLTP) mediates the transfer of phospholipids among atheroprotective high-density lipoproteins (HDL) and atherogenic low-density lipoproteins (LDL) by an unknown mechanism. Delineating this mechanism would represent the first step towards understanding PLTP-mediated lipid transfers, which may be important for treating lipoprotein abnormalities and cardiovascular disease. Here, using various electron microscopy techniques, PLTP is revealed to have a banana-shaped structure similar to cholesteryl ester transfer protein (CETP). We provide evidence that PLTP penetrates into the HDL and LDL surfaces, respectively, and then forms a ternary complex with HDL and LDL. Insights into the interaction of PLTP with lipoproteins at the molecular level provide a basis to understand the PLTP-dependent lipid transfer mechanisms for dyslipidemia treatment.