Purple Brassica oleracea var. capitata F. rubra is due to the loss of BoMYBL2–1 expression

作者：Hayoung Song, Hankuil Yi, Myungjin Lee, Ching‐Tack Han, Jeongyeo Lee, Hye‐Ran Kim, Jong‐In Park, Ill–Sup Nou, Sun‐Ju Kim, Yoonkang Hur · 发表于：BMC Plant Biology · 年份：2018 · DOI：10.1186/s12870-018-1290-9 · 被引用次数：68 · 研究领域：Plant Gene Expression Analysis、Light effects on plants、Phytochemicals and Antioxidant Activities

BACKGROUND: Water-soluble anthocyanin pigments are important ingredients in health-improving supplements and valuable for the food industry. Although great attention has been paid to the breeding and production of crops containing high levels of anthocyanin, genetic variation in red or purple cabbages (Brassica oleracea var. capitata F. rubra) has not yet been characterized at the molecular level. In this study, we identified the mechanism responsible for the establishment of purple color in cabbages. RESULTS: BoMYBL2-1 is one of the regulatory genes in the anthocyanin biosynthesis pathway in cabbages. It is a repressor whose expression is inversely correlated to anthocyanin synthesis and is not detectable in purple cabbages. Sequence analysis of purple cabbages revealed that most lacked BoMYBL2-1 coding sequences, although a few had a substitution in the region of the promoter 347 bp upstream of the gene that was associated with an absence of BoMYBL2-1 expression. Lack of transcriptional activity of the substitution-containing promoter was confirmed using transgenic Arabidopsis plants transformed with promoter::GUS fusion constructs. The finding that the defect in BoMYBL2-1 expression was solely responsible for purple coloration in cabbages was further demonstrated using genomic PCR and RT-PCR analyses of many other structural and regulatory genes in anthocyanin biosynthesis. Molecular markers for purple cabbages were developed and validated using 69 cabbage lines. CONCLUSIO...