Effects of fucoxanthin on autophagy and apoptosis in SGC‐7901cells and the mechanism

作者：Yue Zhu, Jing Cheng, Zhen-Li Min, Tingzi Yin, Rong Zhang, Wei Zhang, Ling Hu, Zhiwen Cui, Chengzhi Gao, Shiqiang Xu, Chunxiang Zhang, Xiamin Hu · 发表于：Journal of Cellular Biochemistry · 年份：2018 · DOI：10.1002/jcb.27022 · 被引用次数：45 · 研究领域：Autophagy in Disease and Therapy、Aquaculture disease management and microbiota、Ginger and Zingiberaceae research

Autophagy and apoptosis are involved in the development of a variety of cancers. Fucoxanthin is a natural compound known to have antitumor effects, so we aimed to explore its effects on autophagy and apoptosis in gastric cancer SGC7901 cells. Specifically, we performed methyl thiazolyl tetrazolium assay, transmission electron microscopy, real-time polymerase chain reaction, Western blot analysis, immunofluorescence assay, and cell apoptosis analysis to clarify the role of fucoxanthin in SGC-7901 cells. Our results indicate that fucoxanthin significantly inhibits the viability of SGC-7901 cells, effectively inducing both autophagy and apoptosis by up-regulating the expressions of beclin-1, LC3, and cleaved caspase-3 (CC3), and by down regulating Bcl-2. Fucoxanthin-induced autophagy also seems to occur before, and may promote apoptosis.