Effects of fertilizations on soil bacteria and fungi communities in a degraded arid steppe revealed by high through-put sequencing

作者：Luhua Yao, Dangjun Wang, Lin‐Woo Kang, Dengke Wang, Yong Zhang, Xiangyang Hou, Yanjun Guo · 发表于：PeerJ · 年份：2018 · DOI：10.7717/peerj.4623 · 被引用次数：37 · 研究领域：Soil Carbon and Nitrogen Dynamics、Biocrusts and Microbial Ecology、Mycorrhizal Fungi and Plant Interactions

BACKGROUND: Fertilization as one of the measures in restoring degraded soil qualities has been introduced on arid steppes in recent decades. However, the fertilization use efficiency on arid steppes varies greatly between steppe types and years, enhancing uncertainties and risks in introducing fertilizations on such natural system to restore degraded steppes. METHODS: ). Soils were sampled from a degraded arid steppe which was consecutively applied with organic and inorganic fertilizers for three years. We analyzed the diversity and abundance of soil bacteria and fungi using high-throughput sequencing technique, measured the aboveground biomass, the soil chemical properties (organic carbon, available and total phosphorus, available and total nitrogen, and pH), and the microbial biomass nitrogen and microbial biomass carbon. RESULTS: In total 3,927 OTU (operational taxonomic units) for bacteria and 453 OTU for fungi were identified from the tested soils. The Ace and Chao of bacteria were all larger than 2,400, which were almost 10 times of those of fungi. Fertilizations had no significant influence on the richness and diversity of the bacteria and fungi. However, the abundance of individual bacterial or fungi phylum or species was sensitive to fertilizations. Fertilization, particularly the phosphorus fertilizer, influenced more on the abundance of the AMF species and colonization. Among the soil properties, soil pH was one of the most important soil properties influencing the...