The Critical Role of IL‐10 in the Antineuroinflammatory and Antioxidative Effects of Rheum tanguticum on Activated Microglia

作者：Jie Meng, Junjun Ni, Zhou Wu, Muzhou Jiang, Aiqin Zhu, Hong Qing, Hiroshi Nakanishi · 发表于：Oxidative Medicine and Cellular Longevity · 年份：2018 · DOI：10.1155/2018/1083596 · 被引用次数：30 · 研究领域：Pharmacological Effects of Natural Compounds、Ginseng Biological Effects and Applications、Phytochemistry and biological activity of medicinal plants

Rheum tanguticum Maxim. ex Balf. ( Rt ), a traditional Tibetan medicine, is known to exert various bioactivities, including anti‐inflammatory and antioxidative activities. The present study was conducted to investigate anti‐inflammatory and antioxidative effects of Rt on activated microglia. Rt (10 μ g/ml) significantly inhibited the mean protein level of interleukin‐1 β (IL‐1 β ) in the organotypic hippocampal slice cultures following treatment with chromogranin A (CGA, 10 nM) and pancreastatin (10 nM), endogenous microglial activators present in senile plaques. Rt also significantly inhibited the expression and production of inflammatory and oxidative molecules, including IL‐1 β , tumor necrosis factor‐ α , and nitric oxide, by cultured microglia after treatment with CGA. These effects of Rt are considered to be mediated by the secretion of interleukin‐10 (IL‐10) from microglia, because neutralizing antibodies against IL‐10 significantly canceled these effects. To explore the causative components of Rt responsible for inducing the secretion of IL‐10, the effects of seven components of Rt on the IL‐10 expression in microglia were examined. Among them, aloe‐emodin (10 μ M) and (+)‐catechin (30 μ M) were able to induce the secretion of IL‐10 from cultured microglia. Therefore, aloe‐emodin and (+)‐catechin are deemed responsible for the antineuroinflammatory and antioxidative effects of Rt through the secretion of IL‐10 from microglia. Accordingly, Rt is considered potentially ...