Cold atmospheric-pressure plasma induces DNA–protein crosslinks through protein oxidation

作者：Li Guo, Yiming Zhao, Dingxin Liu, Zhichao Liu, Chen Chen, Ruobing Xu, Miao Tian, Xiaohua Wang, Hailan Chen, Michael G. Kong · 发表于：Free Radical Research · 年份：2018 · DOI：10.1080/10715762.2018.1471476 · 被引用次数：64 · 研究领域：Plasma Applications and Diagnostics、Plasma Diagnostics and Applications、Surface Modification and Superhydrophobicity

Reactive oxygen and nitrogen species (ROS and RNS) generated by cold atmospheric-pressure plasma could damage genomic DNA, although the precise types of these DNA damage induced by plasma are poorly characterized. Understanding plasma-induced DNA damage will help to elucidate the biological effect of plasma and guide the application of plasma in ROS-based therapy. In this study, it was shown that ROS and RNS generated by physical plasma could efficiently induce DNA-protein crosslinks (DPCs) in bacteria, yeast, and human cells. An in vitro assay showed that plasma treatment resulted in the formation of covalent DPCs by activating proteins to crosslink with DNA. Mass spectrometry and hydroperoxide analysis detected oxidation products induced by plasma. DPC formation were alleviated by singlet oxygen scavenger, demonstrating the importance of singlet oxygen in this process. These results suggested the roles of DPC formation in DNA damage induced by plasma, which could improve the understanding of the biological effect of plasma and help to develop a new strategy in plasma-based therapy including infection and cancer therapy.