Oral Microbiota Community Dynamics Associated With Oral Squamous Cell Carcinoma Staging

作者：Chia‐Yu Yang, Yuan‐Ming Yeh, Haiying Yu, Chia-Yin Chin, Chia‐Wei Hsu, Hsuan Liu, Po-Jung Huang, Songnian Hu, Chun‐Ta Liao, Kai‐Ping Chang, Yu‐Liang Chang · 发表于：Frontiers in Microbiology · 年份：2018 · DOI：10.3389/fmicb.2018.00862 · 被引用次数：380 · 研究领域：Oral microbiology and periodontitis research、Oral Health Pathology and Treatment、Oral health in cancer treatment

Oral squamous cell carcinoma (OSCC) is a highly aggressive cancer and the fourth leading malignancy among males in Taiwan. Some pathogenic bacteria are associated with periodontitis and oral cancer. However, the comprehensive profile of the oral microbiome during the cancer’s progression from the early stage to the late stage is still unclear. We profiled the oral microbiota and identified bacteria biomarkers associated with OSCC. The microbiota of an oral rinse from 51 healthy individuals and 197 OSCC patients at different stages were investigated using 16S rRNA V3V4 amplicon sequencing, followed by bioinformatics and statistical analyses. The oral microbiota communities from stage 4 patients showed significantly higher complexity than those from healthy controls. The populations also dynamically changed with the cancer’s progression from stage 1 to stage 4. The predominant phyla in the oral samples showed variation in the relative abundance of Fusobacteria, Bacteroidetes, and Actinobacteria. The abundance of Fusobacteria increased significantly with the progression of oral cancer from the healthy controls (2.98%) to OSCC stage 1 (4.35%) through stage 4 (7.92%). At the genus level, the abundance of Fusobacterium increased, while the number of Streptococcus, Haemophilus, Porphyromonas, and Actinomyces decreased with cancer progression. Fusobacterium periodonticum, Parvimonas micra, Streptococcus constellatus, Haemophilus influenza, and Filifactor alocis were associated with O...