Facile Chemoenzymatic Synthesis of O‐Mannosyl Glycans

作者：Shuaishuai Wang, Qing Zhang, Congcong Chen, Yuxi Guo, Madhusudhan Reddy Gadi, Jin Yu, Ulrika Westerlind, Yunpeng Liu, Xuefeng Cao, Peng George Wang, Lei Li · 发表于：Angewandte Chemie International Edition · 年份：2018 · DOI：10.1002/anie.201803536 · 被引用次数：40 · 研究领域：Carbohydrate Chemistry and Synthesis、Glycosylation and Glycoproteins Research、Peptidase Inhibition and Analysis

O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms. An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases. In total, 45 O-mannosyl glycans were obtained, covering the majority of identified mammalian structures. Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.