In vivo CRISPR screening unveils histone demethylase UTX as an important epigenetic regulator in lung tumorigenesis

作者：Qibiao Wu, Yahui Tian, Jian Zhang, Xinyuan Tong, Hsin‐Yi Huang, Shuai Li, Hong Zhao, Ying Tang, Chongze Yuan, Kun Wang, Zhaoyuan Fang, Lei Gao, Xin Hu, Fuming Li, Zhen Qin, Shun Yao, Ting Chen, Haiquan Chen, Gong Zhang, Wanting Liu, Yihua Sun, Luonan Chen, Kwok‐Kin Wong, Kai Ge, Liang Chen, Hongbin Ji · 发表于：Proceedings of the National Academy of Sciences · 年份：2018 · DOI：10.1073/pnas.1716589115 · 被引用次数：96 · 研究领域：Epigenetics and DNA Methylation、RNA modifications and cancer、Cancer-related gene regulation

Significance Tumor suppressor genes (TSGs) play important roles in lung cancer initiation, progression, and even metastasis. Here, we take advantage of the clustered regularly interspaced short palindromic repeats/Cas9-mediated screening in vivo technique to identify multiple tumor suppressor genes contributing to lung cancer malignant progression. Using genetically engineered mouse models, we further confirm the tumor-suppressive role of epigenetic regulator UTX and provide therapeutic implications for UTX-deficient lung tumors. Thus, our work provides a systematic screening of TSGs in vivo and demonstrates UTX functions as the important epigenetic regulator in lung tumorigenesis.