CNS Langerhans cell histiocytosis: Common hematopoietic origin for LCH‐associated neurodegeneration and mass lesions

作者：Kenneth L. McClain, Jennifer Picarsic, Rikhia Chakraborty, Daniel Zinn, Howard Lin, Harshal Abhyankar, Brooks Scull, Albert J. Shih, Karen Phaik Har Lim, Olive S. Eckstein, Joseph Lubega, Tricia Peters, Walter Olea, Thomas M. Burke, Nabil Ahmed, John Hicks, Brandon Tran, Jeremy Jones, Robert Dauser, Michael Jeng, Robert A. Baiocchi, Deborah Schiff, Stanton Goldman, Kenneth Heym, Harry Wilson, Benjamin Carcamo, Ashish Kumar, Carlos Rodríguez‐Galindo, Nicholas Whipple, Patrick Campbell, Geoffrey Murdoch, Julia Kofler, Simon Heales, Marian Malone, Randy Woltjer, Joseph F. Quinn, Paul J. Orchard, Michael C. Kruer, Ronald Jaffe, Markus G. Manz, Sérgio A. Lira, D. Williams Parsons, Miriam Mérad, Tsz‐Kwong Man, Carl E. Allen · 发表于：Cancer · 年份：2018 · DOI：10.1002/cncr.31348 · 被引用次数：138 · 研究领域：Histiocytic Disorders and Treatments、CNS Lymphoma Diagnosis and Treatment、Tuberous Sclerosis Complex Research

BACKGROUND Central nervous system Langerhans cell histiocytosis (CNS‐LCH) brain involvement may include mass lesions and/or a neurodegenerative disease (LCH‐ND) of unknown etiology. The goal of this study was to define the mechanisms of pathogenesis that drive CNS‐LCH. METHODS Cerebrospinal fluid (CSF) biomarkers including CSF proteins and extracellular BRAF V600E DNA were analyzed in CSF from patients with CNS‐LCH lesions compared with patients with brain tumors and other neurodegenerative conditions. Additionally, the presence of BRAF V600E was tested in peripheral mononuclear blood cells (PBMCs) as well as brain biopsies from LCH‐ND patients, and the response to BRAF‐V600E inhibitor was evaluated in 4 patients with progressive disease. RESULTS Osteopontin was the only consistently elevated CSF protein in patients with CNS‐LCH compared with patients with other brain pathologies. BRAF V600E DNA was detected in CSF of only 2/20 (10%) cases, both with LCH‐ND and active lesions outside the CNS. However, BRAF V600E + PBMCs were detected with significantly higher frequency at all stages of therapy in LCH patients who developed LCH‐ND. Brain biopsies of patients with LCH‐ND demonstrated diffuse perivascular infiltration by BRAF V600E + cells with monocyte phenotype (CD14 + CD33 + CD163 + P2RY12 ‐ ) and associated osteopontin expression. Three of 4 patients with LCH‐ND treated with BRAF‐V600E inhibitor experienced significant clinical and radiologic improvement. CONCLUSION In LCH‐N...