Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms

作者：Xiaofei Xie, Hong-juan Chu, Yufen Xu, Liang Hua, Zhouping Wang, Ping Huang, Hong-Ling Jia, Li Zhang · 发表于：Cardiology Journal · 年份：2018 · DOI：10.5603/cj.a2018.0032 · 被引用次数：32 · 研究领域：Kawasaki Disease and Coronary Complications、Coronary Artery Anomalies、Inflammasome and immune disorders

BACKGROUND: To study the protein profile of the serum exosomes of patients with coronary artery aneurysms (CAA) caused by Kawasaki disease (KD). METHODS: Two-dimensional electrophoresis (2-DE) was used to identify proteins from the exosomes of serum obtained from children with CAA caused by KD, as well as healthy controls. Differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight/timeof-flight mass spectrometry (MALDI-TOF/TOF MS) analysis. RESULTS: Thirty two differentially expressed proteins were identified (18 up-regulated and 14 downregulated) from serum exosomes of children with CAA and were compared to healthy controls. The expression levels of 4 proteins (TN, RBP4, LRG1, and APOA4) were validated using Western blotting. Classification analysis and protein-protein network analysis showed that they are associated with multiple functional groups, including host immune response, inflammation, apoptotic process, developmental process, and biological adhesion process. CONCLUSIONS: These findings establish a comprehensive proteomic profile of serum exosomes from children with CAA caused by KD, and provide additional insights into the mechanisms of CAA caused by KD.