Membrane protein MHZ3 stabilizes OsEIN2 in rice by interacting with its Nramp-like domain

作者：Biao Ma, Yang Zhou, Hui Chen, Sijie He, Yi‐Hua Huang, He Zhao, Xiang Lu, Wan‐Ke Zhang, Jinhuan Pang, Shou‐Yi Chen, Jin‐Song Zhang · 发表于：Proceedings of the National Academy of Sciences · 年份：2018 · DOI：10.1073/pnas.1718377115 · 被引用次数：54 · 研究领域：Postharvest Quality and Shelf Life Management、Plant responses to water stress、Plant Stress Responses and Tolerance

overexpression elevates the abundance of both wild-type and mutated OsEIN2 proteins, suggesting that MHZ3 is required for proper accumulation of OsEIN2 protein. The association of MHZ3 with the Nramp-like domain is crucial for OsEIN2 accumulation, demonstrating the significance of the OsEIN2 transmembrane domains in ethylene signaling. Moreover, MHZ3 negatively modulates OsEIN2 ubiquitination, protecting OsEIN2 from proteasome-mediated degradation. Together, these results suggest that ethylene-induced MHZ3 stabilizes OsEIN2 likely by binding to its Nramp-like domain and impeding protein ubiquitination to facilitate ethylene signal transduction. Our findings provide insight into the mechanisms of ethylene signaling.