Prognostic significance of the infiltration of CD163 + macrophages combined with CD66b + neutrophils in gastric cancer

作者：Xiaopei Huang, Ya‐min Pan, Jun Ma, Zhengchun Kang, Xiaowen Xu, Yan Zhu, Ji‐Kuai Chen, Wei Zhang, Wenjun Chang, Jiangbo Zhu · 发表于：Cancer Medicine · 年份：2018 · DOI：10.1002/cam4.1420 · 被引用次数：48 · 研究领域：Immune cells in cancer、Cancer Cells and Metastasis、Immune Cell Function and Interaction

Abstract The polarization of tumor‐associated macrophages (TAMs) and tumor‐associated neutrophils (TANs), especially from the antitumoral phenotype to the protumoral phenotype under certain conditions, has an important influence on the progression of tumors. However, the interactions and combined prognosis of these cells are poorly known. Here, we detected the infiltration of CD68 + TAMs, CD163 + TAMs, and CD66b + TANs in the specimens from 662 patients with GC by immunohistochemistry. The results showed that the infiltration of each of CD163 + , CD68 + , and CD66b + cells in GC tissue was significantly increased and independently associated with GC prognosis. Strong collinearity (r = 0.690, P < 0.001) was found between the infiltration of CD163 + and CD68 + cells in GC, and multivariate Cox analysis confirmed the infiltration of CD163 + cells was a better predictor for prognosis than that of CD68 + cells. The combination of the infiltration of CD163 + and CD66b + cells provided more accurate survival prediction than any individual marker. Patient subgroups with CD66b low CD163 low (hazard ratio (HR) = 2.161; 95% confidence interval (CI) = 1.266–3.688; P < 0.001), CD66b high CD163 high (HR = 3.575; 95% CI = 2.155–5.933; P < 0.001), and CD66b low CD163 high (HR = 7.514; 95% CI = 4.583–12.312; P < 0.001) were gradually associated with shorter DFS when compared with the subgroup with CD66b high CD163 low . The similar result was also for DSS among the subgroups. More...